OF THE COCOANUT DURING GERMINATION 343 
On the opposite page we give a microphotographic view of 
edestin crystals from our second preparation Although not the 
purest, we have selected this preparation for this purpose because 
its crystals are mostly rhombohedra. These forms rarely occur in 
abundance in edestin precipitates, octahedra being more commonly 
obtained. Most of the larger masses among the crystals shown 
here are ‘‘ rounded” octahedra; not in perfect focus because they 
are thicker than the rhombohedra. The smaller particles consist 
of globular matter and crystal pieces. 
The crystals given in Fig. ¢ were photographed for us by the 
writer’s colleague, Dr. Edward Leaming, who cordially gave us 
the benefit of his large experience. We wish here again to extend 
to Dr. Leaming our sincere thanks for his valuable assistance. 
_ That the substance under discussion is edestin is further shown 
by the results of analysis. We append our results for nitrogen 
content, as determined by the Kjeldahl method, calculated for ash- 
free substance : 
PERCENTAGE OF NITROGEN IN CocoA EDESTIN 
a NN 
Preparation, I | 2 | 3 | 4 | 5 
eee | 
Analytic results, | 17.87 17.85 17.66 | $14 17. 15,25 
ace Se seas A 17.69 | 18.21 | 18.20 
t. 37239 17.91 17.78 | 1838 | 18.28 
ieage —|2| | |S 
Sa 7.60. | a7 | 18.18 ee 124 
Ash. fp Oakes | 0.13 tet Ree 150.4 1.84 
Preparations 1, 2 and 3 contained amorphous material, possibly some of 8 
gummy matter referred to on page 342, in spite of our efforts to completely remove it. 
Preparations 4 and 5 were obtained from 1 and 3 by further treatment with diastase 
and by recrystallization by dialysis from 10-per-cent. salt solution. They were practi- 
cally wholly crystalline. 
The above results show that the globulin separated from the 
_ Cocoanut by the methods here employed is edestin.* 
This same proteid of the cocoanut was examined by Ritt- 
hausen, + who termed it conglutin without really identifying it with 
that substance. His analyses gave it a content of nitrogen of 
17:87-17.91 per cent. Chittenden and Setchell ¢ referred to it by 
* The edestin from barley contains 18.10 per cent, N. That from maize 18.12 
Per cent.; from rye, 18.19 per cent.; wheat, 18.39 per cent. Osborne: Journal of the 
American Chemical Society, 17: 547. _ 1895. 4 ‘a 8. 1880 
T Ritthausen : Jahresbericht iiber die Fortschritte der Thier-Chemie, to ie cas Nemnere 
t Chittenden and Setchell : Quoted by Chittenden, Digestive Proteolysis, 32. 1895. 
