344 Kirkwoop AND Gigs: CHEMICAL STUDIES 
the name of phytovitellin. The composition they gave for it is in 
general accord with that of edestin (nitrogen content = 18.40 per 
cent.), and as they obtained it partly crystallized in octahedra, Os- 
borne * has lately suggested that the substance is edestin. The 
results we have obtained confirm Osborne’s deduction. 
The proteose to which we have already alluded was obtained 
from the globulin filtrate. The latter was freed from traces of 
globulin by the coagulation method, the hot filtrate evaporated to 
a small bulk on the water-bath and the proteose precipitated and 
purified by the usual method. + About four grams were obtain- 
able from fifteen nuts. The product contained both proto and 
deutero forms. Some heteroproteose was also detected in the 
products formed on dialysis and a trace of dysprotose was 
obtained. 
The following results for nitrogen content in the ash-free sub-— 
stance were obtained by the Kjeldahl method : 
PERCENTAGE OF NITROGEN IN CoCcOA PROTEOSE 
—_— =e 
Preparation. I 2 | 3 | General Ae 
Analytic results. 18.67 18.48 18.57 | 
; 18.50 18.46 18.61 | 
18.58 18.40 8.5600) 0 
Average. 18.58 18.45 18.57 | SS ee 
Ash. 714 pon) ce eee 159 ae 
These results differ only slightly from those reported by Chit- 
tenden and Setchell.{ This difference may be explained by the 
fact that mixtures of proteoses have been analyzed in each case 
by Chittenden and Setchell,and by us. Their preparation of Pro 
teose contained 18.25 per cent. of nitrogen. : 
In his volume entitled Digestive Proteolysis, Chittenden BN 
the analytic results for eleven different proteids and the proteoses 
derived from them (page 67). For seven of these the nitrogen of 
the corresponding proteose is somewhat higher than that of the 
original proteid. Analysis of our own preparations has shown irs 
percentage of nitrogen to be greater in the proteose than 10 the 
globulin, a result in accord with the majority rule just noted. 
* Osborne : Journal of the American Chemical Society, 18: 13. 1896. 
+ MacDougal : Practical Text-book of Plant Physiology, 164, 1901. 895- 
{ Chittenden and Setchell : Quoted by Chittenden, Digestive Proteolysis, 32°! 
