356 - Krrxwoop AND GIEs: CHEMICAL STUDIES. 
ing weights of the fresh parts were very carefully taken; the data 
for nitrogen were determined by the Kjeldahl method.* 
Weight Percentage of Percentage 
in Grams. Total Weight. of Nitrogen. 
Cotyledon, 196 28.1 
Central part, OnE 
Cortical layer, 0.3% 
Endosperm, 155 22.2 
Distal portion, 0 65 
Proximal portion, 0.93 
Shell, 161 23.0 
Stem, 16 2.3 
Lower part, oT 
** Root crown,”’ 0.53 
Roots, 41 5.9 
Inside of husk, 0.27 
Outside of husk, 0.54 
Petioles and young leaves, 58 8.3 0.29 
Petioles, 0.39 
Old leaves and petioles, 74 10.2 045 
TOTAL WEIGHT, 698 
Shell and contents, 512 73-4 
Whole plant, 186 26.6 
Cotyledon and endosperm, 351 50.3 
The tables on pages 354-355 give all our results for general 
composition of the parts of the germinated nut. Numerous 
deductions may be drawn from these results, as to growth and 
metabolism. 
The central part of the cotyledon, with its vascular network, 
contains more water and less solid matter than any other part of 
the germinated nut. The proportion of solid substance in it in- 
creases toward the corrugated epithelium, being greatest in the 
“neck,” where the structure is fibrous and woody. 
That the absorbing organ completely takes up the milk is very 
evident from the way it fills the cavity and from its own composition, 
but it is likewise apparent from our results that water is also with- 
——e 
* The husk was not weighed because it was decayed underneath and water-logget 
Nitrogen was not determined in the shell because its substance remains unaltered a 
ing germination. 
+ The methods of determination were the same as those used previously- 4 
roots, outside of the husk, which had been in the wet soil, were hurriedly ri pe oe 
water to remove inorganic matter then wiped dry with a towel and at once 
thin cross sections for analysis. 
