126 GERM-CELL CYCLE IN ANIMALS 



lying the terminal cap (Fig. 39, t. c) there are a large 

 number of spermatogonia not yet contained in cysts. 

 All stages in cyst formation may be observed here 

 not only in larval testes but also in those of pupse 

 and adults. The youngest spermatogonia are those 

 lying near the terminal cap. Figure 40, A shows a 



few cells of the 

 terminal cap (t.c), 

 some of the neigh- 

 boring spermato- 

 gonia (spg), and 

 several of the epi- 

 thelial cells (ep) 

 that are scattered 

 about among the 

 spermatogonia. 

 Cysts are formed 



riG. 39. Leptinotarsa decemhneata. Longi- 



tudinal section through testis of full-grown toward the edge 

 larva. c = cavity ; cy = region of cysts ; . , 



s.d = sperm duct ; sg = region of spermato- OI tne 



gonia ; sp = region of spermatozoa ; t.c = gonial mass 

 terminal cap. 



from the terminal 



cap, and Fig. 40, A to G represent certain of the 

 stages observed. The spermatogonia divide ap- 

 parently exclusively by mitosis. A well-developed 

 spindle is formed and this persists after the cell wall 

 has separated the two daughter cells. The spindle 

 fibers which are at first perfectly distinct (Fig. 40, B) 

 unite into a compact strand (Fig. 40, C) which 

 stains dense black in iron hsematoxylin after fixa- 

 tion in Carney's fluid. In many cases it was im- 

 possible to distinguish an intervening cell wall 



