PLATE III. 



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Cultivation of amosbse by the "moist-air-chamber ' method. The most rapid growth of 

 amoebae is obtained on a jelly surface which is over (not in contact with) water. The 

 diagram shows a culture in a small Petri dish over water in a larger dish. Auxetics and 

 augmentors can be added to the jelly and their action on the amoebae observed 



A modification of the above method. The jelly film is on a microscope slide, which is inverted 

 (resting on corks) over the water. 



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