

LIVE SLIDE 21 



bottle can be hung by means of a piece of wire. 

 The plate is covered over with a sheet of fine 

 calico having a circular hole in the centre just large 

 enough to permit of a cover-glass resting on the 

 plate with its edges almost touching the cloth all 

 round. Two narrow strips of cigarette paper are 

 placed on opposite sides of the aperture under the 

 cover-glass to prevent this from actually pressing 

 on the slide. This support is absolutely necessary 

 when examining amoebae, but can be dispensed 

 with in the case of smaller bodies such as flagellates, 

 etc. Water or the solution to be employed is 

 placed in the bottle, and is slowly conveyed by 

 capillarity along a piece of lamp wick to the cloth, 

 which is in this way kept continually moist. The 

 amoebae (free forms or cysts) are put into a drop of 

 the solution on the cover-slip, which is then inverted 

 and allowed to rest on the slide in the way described. 

 This apparatus has been in constant use for weeks 

 without drying up of the cloth or of the solution 

 under the cover-glass, all that is necessary being to 

 put a little fresh solution in the bottle every few 

 days. Either free amcebaa or cysts may be examined 

 in this way, and when required, a jelly may be 

 interposed between the slide and the cover-glass as 

 described above. 



METHOD B. EXAMINATION ON A JELLY SURFACE 



This is an adaptation of the principle employed 

 in slope cultures in test-tubes with water of con- 

 densation, but has the advantages that the amoebae 

 can be examined at any time without interference 

 and there is no danger of drying up. This " moist- 

 iv 2* 



