74 ENCYSTMENT AND EXCYSTATION OF AMCEBA 



on one of the tubes (E), in which no excystation 

 had taken place, and the amoebae excysted within 

 twenty-four hours. 



All the tubes were tested for sterility by means 

 of agar slopes and gelatine stabs, and, as a further 

 precaution, the slopes employed in the experiments 

 contained one-third part of gelatin, which showed 

 no signs of liquefaction. 



TABLE II. THE ACTION OF NORMAL HORSE SERUM IN 

 CAUSING EXCYSTATION OF AMOEBA CYSTS, AND THE EFFECT 

 OF AUXETICS AND KlNETICS IN INDUCING REPRODUCTION 

 OF AMOEBAE 



Cultures of amoeba on liquid food in the presence 

 of auxetics and kinetics. The next steps were 

 carried out without any dead bacteria as follows : 

 To 5 cc. of sterile 2-per-cent. agar jelly in a test- 



