416 PROCEEDINGS OF THE 



William Buttemer were balloted for and duly elected members of 

 the Club. 



The list of donations to the Club was read and the thanks of 

 the members voted to the donors. 



Mr. W. R. Traviss exhibited a number of specimens of insects 

 in amber. 



Mr. A. E. Hilton read " Some Notes on the Cultivation of 

 Plasmodia of Badhamia utricularis" He said that a free- 

 flowing mass of naked and almost undifferentiated protoplasm, 

 such as we have in a plasmodium of B. utricularis, suggests 

 opportunities for biological experiments with unusual promise of 

 success. 



The chief purpose of this paper, Mr. Hilton said, was to place 

 on record the results of experiments made during the last few 

 months, which suggest a method of continuous cultivation of 

 plasmodia of B. utricularis at once simple and practicable. 



The President said they were very much obliged to Mr. Hilton 

 for his very interesting and practical paper, which he should find 

 of great value to himself, as he had hitherto had great difficulty 

 in feeding this organism. He hoped the methods described would 

 come into general use for laboratory work, where the plasmodium 

 was very useful as an illustration. He should like to ask 

 Mr. Hilton if he had tried how long he could keep the plas- 

 modium in a dry state on the blotting-paper. Mr. J. J. Lister 

 at Cambridge used to feed it on fungus, but this was sometimes 

 difficult to get. He hoped that many members of the Club would 

 experiment in the manner suggested. 



Replying to several questions, Mr. Hilton said the dried 

 sclerotium is capable of reviving after at least three years; but 

 it must be kept dry, and never allowed to become damp. After 

 so long a period, it might take four or five days to recover. He 

 could not say if it were possible to cultivate the plasmodium form 

 from sporangia. A difference in colour has been noticed in 

 specimens cultivated on plain bread compared with specimens 

 fed on the special mixture. The former are a lighter yellow 

 than the latter ; but various shades of yellow are present even in 

 one plasmodium. He had found a constant temperature of about 

 50 F. the best. 



A very hearty vote of thanks was accorded to Mr. Hilton for 

 his paper. 



