276 JOHNS HOPKINS UNIVERSITY MORPHOLOGICAL MONOGRAPHS. 



from the fertilized egg, and since it presents many points of resemblance 

 to asexual multiplication, especially to the process of budding, I have 

 proposed the name follicular budding for this sort of reproduction. The 

 fundamental characteristics of follicular budding, which have impelled 

 me to the use of this name, are the proliferation of the follicle cells, and 

 their contribution to the construction of the embryo." 



My own observations seem to me to prove that the nuclei of many of 

 the visceral follicle cells become converted into food for the developing 

 blastomeres, while Salensky holds that the blastomeres degenerate and 

 supply food for the follicle cells, but so far as our observations go there 

 is no very essential disagreement. 



In his account of the embryo of Salpa pinnata, at about the same 

 stage as my figure of the embryo Salpa hexagona, Plate XI, Fig. 2, he 

 says (5), page 99, " I will here call attention to the noteworthy changes in 

 the histological condition of the blastomeres. Their protoplasm, which 

 at all the earlier stages was finely granular and almost transparent, now 

 falls into small, irregular patches, which are sometimes around the 

 nucleus and sometimes at the periphery of the cells. When I first dis- 

 covered this peculiar degeneration of the protoplasm, I attributed it to the 

 action of the preserving or staining fluids. It is, however, so constant at 

 certain early stages, after the first steps in segmentation, and is so inde- 

 pendent of the method of preservation, that I am convinced that this 

 alteration of the protoplasm is a normal phenomenon in the develop- 

 ment of salpa. Briefly stated, the protoplasm falls into a central mass 

 around the nucleus and a number of peripheral polygonal portions." 



His statement, page 100, that while these changes are taking place 

 in the blastomeres, the follicle cells (gonoblasts) present nothing note- 

 worthy except their vigorous multiplication, shows that he failed to 

 study the stages shown in my Plate XLII, Figs. 1 and 2, for at this time 

 the changes in the follicular nuclei are eminently noteworthy. 



His figures of the embryo of Salpa democratica are most instructive, 

 and seem to prove, almost as clearly as my own, that the bodies in the 

 protoplasm of the blastomeres are migrating follicular nuclei. 



I have already said that in his Taf. 27, Fig. 2, dt, he has. drawn four 

 of the peculiar pear-shaped migrating nuclei, and in Taf. 27, Fig. 1, dt, A, 

 he shows a great number of bodies of the same size and shape in the pro- 

 toplasm of the blastomeres, and gives, on page 379, the following account 

 of them: "The protoplasm of the blastomeres of Salpa democratica is, 

 in contrast to that of other species, perfectly homogeneous. It is note- 



