THE ANTIBERIBERI VITAMINE 



181 



obtained from yeast. The dose of crude fraction used was 4 to 8 

 mgm., and the preparation was administered intramuscularly. The 

 product gave a strong uric acid and phenol reaction with the Folin- 

 Macallum reagents (486). 



FIG. 45. PIGEON BEFORE TREATMENT 



FIG. 46. SAME PIGEON (CF. FIG. 45) AFTER THREE HOURS, WITH 8 MGM. 



YEAST VITAMINE 



This 2.5 grams of crude fraction was then recrystallized from 

 dilute alcohol, and gave, at first, 1.6 grams of a substance crystallizing 

 in microscopic needles. Even after several recrystallizations, the 

 melting point could not be raised above 229 C. (corrected). The 

 first crystallization still gave the reactions mentioned above; only 



