184 



THE VITAMINES 



Mixed with the nicotinic acid from rice polishings, it showed the same 

 melting point, and the picrates behaved similarly. 



The mother liquor of the picrate precipitate, which gave a strong 

 uric acid and phenol reaction, was shaken out in acid solution with 

 ether in order to remove the picric acid. After elimination of the 

 hydrochloric acid, the solution was slowly evaporated, yielding 

 0.4 gram of a substance melting at 210 C. and when recrystallized 

 from dilute alcohol to constant melting point, melted at 222 to 223 C. 

 (microscopic needles, somewhat more soluble in water than substance 

 I). The pure substance no longer gave the uric acid nor the Millon 

 reaction, but the phenol reaction was still positive. For analysis, 

 one sample was dried in the vacuum dessicator over sulphuric acid at 

 ordinary temperature; another was dried in vacuum at 100 C. and 

 another, at 115 C. The substance dried over sulphuric acid gave 

 the following figures: 



3.559 mgm. gave 7.745 mgm. CO 2 and 1.21 mgm. H 2 O; 59.35 per cent C; 3.80 



per cent H. 

 3.445 mgm. gave 0.375 cc. N (706 mm. 17C.), 11.90 per cent N. 



Dried in vacuum at 100C. 

 4.172 mgm. gave 9.065 mgm. CO 2 and 1.535 mgm. H 2 O; 59.26 per cent C; 4.12 



per cent H. 

 2.716 mgm. gave 0.295 cc. N and (711 mm. 15C.) 12.04 per cent N. 



Dried in vacuum at 115C. 



3.694 mgm. gave 7.99 mgm. CO 2 and 1.22 mgm. H 2 O; 58.99 per cent C; 3.70 per 

 cent H. 



These figures correspond equally well to two formulas, though 

 somewhat better to the second. 



To recapitulate briefly the yeast fractionation, we have been able 

 to isolate three different substances from the vitamine fraction, 

 which gives a strong uric acid and phenol reaction. 



