vi vrv fa 



ON THE BEHAVIOR OF BUFO AND RANA TOWARD 



COLLOIDAL DYES OF THE ACID AZO 



GROUP (TRYPAN BLUE AND DYE NO. 161) 



I. WITH REFERENCE TO THE PORTAL OF ENTRY OF THE DYE, AND 

 THE CAUSES WHICH UNDERLIE THE INITIATION OF THE PROC- 

 ESS BY WHICH COLLOIDAL DYE PARTICLES ARE STORED 

 IN THE CYTOPLASM OF CERTAIN TYPICAL CELLS OF THE 

 EMBRYO, AND II. WITH REFERENCE TO THE DE- 

 VELOPMENT OF THE LYMPHATIC SYSTEM 



CHARLES F. W. McCLURE 



Laboratory of Comparative Anatomy, Princeton University 



CONTENT'S 



1. Introduction 2 



2. Methods 5 



3. On the determination of the earliest stage of larval development in Bufo 



and Rana, at which certain typical tissues are first observed to react 

 toward colloidal acid dyes 7 



4. Reactions of the tissues in Bufo and Rana toward colloidal acid dyes, as 



observed in larvae when killed at the critical ontogenetic stage 12 



5. Experiments to determine the rate at which the storage of dye granules 



may be accomplished in larvae of Bufo which have been placed in a solu- 

 tion of colloidal acid dye at the critical ontogenetic stage 16 



6. Experiments which show that the storage of dye granules may be accom- 



plished in a relatively short time, when larvae are placed in solutions of 

 colloidal acid dyes at an advanced stage of development 18 



7. Experiments which show that the method of vitally staining typical tis- 



sues by immersing young larvae in solutions of colloidal acid dyes, can 

 also be applied to year-old larvae of R. catesbiana and to adult anura 22 



8. Experiments to determine the portal of entry of the dye 25 



A. Suspension of only a portion of the body in a solution of colloidal 

 acid dye 26 



B. Feeding experiments on adult frogs and toads 34 



9. A comparison of the reactions of the tissues toward colloidal acid dyes as 



observed in experiments concerned with the portal of ent ry of t he dye ... 37 



1 



MEMOIR NO. 8 



