8 



CHARLES F. W. McCLURE 



shown that if these twelve larvae had been killed in the morning 

 of June 8, instead of June 9, no indication of dye granules would 

 likely have been observed in any cell of the body. 



TABLE 1 

 Bufo lentiginosus 



An examination of serial sections of five (5) of these larvae 

 killed on June 5, five (5) killed on June 6, eleven (11) killed on 

 June 7, eleven (11) killed on June 8 and two (2) killed on June 9, 

 showed that no granules had been stored in the cytoplasm of any 

 cell in these thirty-four (34) larvae. 



Fourteen (14) larvae were killed on June 9 (ninth day after 

 fertilization) and sectioned. In two (2) individuals, as stated 

 above, no dye granules were observed in the cytoplasm of any 

 cell. In the remaining twelve (12) larvae, however, dye gran- 

 ules were invariably met with in the cytoplasm of the cell in 

 certain typical tissues (table 1, column V). 



In all of the larvae concerned in this experiment which were 

 killed after June 9, six (6) on June 10, five (5) on June 11, five 



