30 CHARLES F. W. McCLURE 



hour and forty-five minutes. No dye was stored in the liver and 

 kidneys or in any other tissues as far as examined. 



6) An adult of R. palustris was suspended for short intervals 

 daily from July 5 to July 8, inclusive, in a 1 : 100 solution of Niagara 

 blue (dye No. 161), and killed on July 9. The actual length of time 

 the frog was suspended in dye during a period of four days was 

 three hours and forty-five minutes. 



On removal of skin the muscles of the abdomen presented a 

 greenish-blue appearance, while the muscles and joints of the 

 hind limbs were stained a deep blue. The walls of the oral cavity 

 and pharynx were deeply stained. The walls of the stomach and 

 rectum were markedly bluish, but the small intestines remained 

 unstained. 



Sections showed the unmistakable presence of dye granules in 

 the stellate cells of the liver and in the epithelium of a few tubules 

 in the kidney. Not a trace of dye was observed in sections of the 

 testis or spleen. Sections of the stomach showed the submucosa 

 to be lightly, though diffusely stained. Mononuclear cells loaded 

 with dye granules were observed in the mucosa of the stomach. 



c) A young adult of R. catesbiana was suspended for short 

 intervals daily from July 19 to July 23, inclusive, in a 1:100 

 solution of trypan blue and killed on July 24. The actual length 

 of time the frog was suspended in dye during a period of five 

 days was fourteen hours and forty-five minutes. 



Serial sections were made of the liver, kidneys and spleen. 

 Dye granules were stored in the stellate cells of the liver capil- 

 laries and in the epithelium of a few tubules in the kidney. 

 Large deeply stained mononuclear leucocytes containing dye 

 granules were found in the intertubular tissue of the kidneys and 

 more lightly stained ones in the spleen. 



d) An adult of R. palustris was suspended for- short intervals 

 daily from July 19 to 24, inclusive, in a 1 :150 solution of trypan 

 blue and killed on July 25. The actual length of time the frog 

 was suspended in dye during a period of six days was twenty-one 

 hours and twenty-five minutes. 



The muscles of the legs and abdomen were decidedly bluish in 

 appearance, also the joints of both fore and hind limbs. The 



