32 CHARLES F. W. McCLURE 



TABLE 8 

 Partial immersion of body of adult toads in solutions of colloidal acid dyes 



slight. It is important to note, however, as observed in a number 

 of other experiments, that the mononuclear cells and the epithe- 

 lium of the kidney appear to store the dye prior to the stellate 

 cells of the liver, in all cases where the portal of entry of the dye 

 is restricted to the integument. 



Experiment 14 (table 8). (Partial immersion of body of adult 

 toads in solutions of colloidal acid dyes) . 



In the following experiments only the hind legs and posterior 

 half of the body were suspended in the dye. 



a) A young adult of B. lentiginosus was suspended for short 

 intervals daily between July 7 and July 9, inclusive, in a 1 : 100 

 solution of Niagara blue (dye No. 161) and killed on July 10. 

 The actual length of time the toad was suspended in dye during a 

 period of three days was three hours and thirty-seven minutes. 



The muscles, joints and viscera did not show the slightest sign 

 of being diffusely stained by the dye. Examination of sections 

 showed that a doubtful trace- of dye had been stored in a very few 

 of the stellate cells of the liver capillaries, and that the epithelium 

 of a few tubules in the kidney had stored the dye. One would 

 likely have overlooked the presence of dye granules in both the 

 liver and kidney, if serial sections of the entire organs had not 

 been made. The most interesting feature of this experiment is 

 that deeply stained mononuclear leucocytes containing dye gran- 



