BEHAVIOR OF ANURA TOWARD COLLOIDAL DYES 33 



ules were occasionally found in the intertubular tissue of the 

 kidney, which shows that these cells must have been actively 

 phagocytic prior to the Kupffer cells. No dye was stored in any 

 cell of the spleen. 



6) A young adult of B. lentiginosus was suspended for short 

 intervals daily between July 23 and July 25, inclusive, in a 1 : 100 

 solution of trypan blue and killed on July 26. The actual length 

 of time the toad was suspended in the dye during a period of three 

 days was three hours and forty minutes. 



On removing the skin some of the joints appeared slightly 

 bluish but the muscles were unstained. 



A mere trace of dye was stored in a few of the stellate cells of 

 the liver capillaries and in the epithelium of a few of the tubules 

 in the kidney. Deeply stained mononuclears containing dye 

 granules were found in the intertubular tissue of the kidneys. 



c) A young adult of B. lentiginosus was suspended for short 

 intervals daily between July 23 and July 26, inclusive, in a 1 : 100 

 solution of trypan blue and killed on July 27. The actual length 

 of tune the toad was suspended in the dye during a period of four 

 days was five hours and thirty minutes. 



On removal of skin the knee joints appeared slightly bluish but 

 the muscles and viscera were unstained. 



A mere trace of dye was observed in a few of the stellate cells of 

 the liver capillaries in the form of very minute and pale bluish 

 granules. In teased preparations of the kidney some of the tubules 

 were slightly bluish and sections showed that dye granules were 

 stored in the epithelium of a few tubules. Deeply stained mono- 

 nuclears containing dye granules were occasionally found in the 

 intertubular tissue of the kidney. 



d) An adult of B. lentiginosus was suspended for short intervals 

 daily from July 16 to July 24, inclusive, hi a 1:200 solution of 

 Niagara blue (dye No. 161) and killed on July 25. The actual 

 length of time the toad was suspended in dye during a period of 

 nine days was twenty-five hours and thirty-seven minutes. 



The muscles of the body and walls of the intestinal canal were 

 quite deeply stained. The vessels in the mesentery, the joints 



MEMOIR NO. 8 



