16 CHARLES F. W. McCLTJEE 



each curve in figure 5 indicate the order in which the experi- 

 ments were made. For example, the first transfer (curve 1) 

 was made from water at 10 C., in which the frog had pre- 

 viously remained for a considerable time, to water at 17 C. 

 The second transfer (curve 2) was made from water at 13 C. 

 to water at 8C., and so on. Between experiments 6 and 9 

 two desiccation recovery curves (McClure, '25, fig. 1), were 

 made, based on the reactions of the same frog. These are 

 not included in the figure. The odd numbers (curves 1, 3, 5, 

 9, and 11 in fig. 5) represent the behavior of the frog when 

 transferred from water at a low to water at a higher tem- 

 perature. The even numbers (curves 2, 4, 6, 10, and 12 in 

 fig. 5) represent the behavior of the frog when transferred 

 from water at a high to water at a lower temperature. Had 

 it been practical to do so, the behavior of this frog would 

 have been graphically represented in one curve. In the pres- 

 ent case the zero point on the ordinate marks the beginning 

 of each transfer. This series of experiments extended over a 

 period between March 26th and April 26th, and was made on 

 a frog obtained from a dealer in February. During the course 

 of the last experiment (curve 12} the frog contracted red-leg 

 disease, and its gain in weight then became continuous 

 (McClure, I.e., '25). This last experiment (curve 12) ex- 

 tended over a period of 170 hours, at the end of which time 

 the gain in weight of the frog amounted to 32.8 per cent a 

 gain not graphically represented in the figure. Since a con- 

 tinuous increase in body weight in the frog is coincident with 

 the appearance of oedema, the writer deemed it worth while 

 to make an analysis of these fluctuations observed both in the 

 skin and in the living frog. 



IV. THE BEHAVIOE OF GLOVES OF FBOG'S SKIN, WITH THE INSIDE 

 OF THE SKIN TUENED OUTWARD, WHEN FILLED WITH RINGEE'S 

 SOLUTION APPROXIMATELY ISOTONIC WITH FEOG'S BLOOD AND 

 SUSPENDED IN THE SAME SOLUTION 



When skin gloves, with the inside of the skin turned out- 

 ward, are filled with isotonic Ringer 's solution and suspended 

 in the same solution, there is, as numerous investigators have 



