LYMPH FLOW 



21 



perature (17C.). As previously stated, during the first 

 hour the gain in weight of the glove may have resulted partly 

 from differences in osmotic pressure of the fluid in the skin 

 and that of the solution in which the glove was suspended; 

 but it would be difficult to explain its succeeding behavior as 

 an osmotic reaction. The loss in weight of the glove during 

 the second hour indicates that the hydration capacity of the 

 skin had previously been satisfied; and the fact that a loss 

 in weight regularly occurred when the glove was suspended 

 in Ringer at 27C., and a gain when it was suspended in 

 Ringer at 17C., indicates that the gain and the loss in weight 

 were due not to osmotic pressure, but rather to the gain and 

 the loss of water by tissue colloids. 



Gam 5 



Pfrcent o 



Loss s 



\ JriSi'deif Sifin out 4J7 A 



Hours 



I 3 



B 9 iO 



The control of this experiment (457, fig. 7), was the skin 

 glove from the left hind leg of the same frog, with the outside 

 of the skin turned outward; this skin was filled with Ringer's 

 solution approximately isotonic with frog's blood, and was 

 then suspended at different temperatures in the same solu- 

 tion. The behavior shows that during the first six hours 

 there was not a continuous transport of water through the 

 skin and that even at the end of twenty-four hours' suspen- 

 sion the gain in weight of the glove was insignificant. Since 

 in the control the normal direction in which water is trans- 

 ported through the integument is from without inward, we 

 may infer that in the control the loss in weight observed dur- 

 ing the second hour is due to a loss of water by the tissue 



