50 CHARLES F. W. McCLUKE 



itself in a direction opposite to that of osmotic pressure was 

 less in experiment 571 than in experiment 533. 



In the above experiments we have shown how the driving 

 force of osmosis may be modified by the presence of K^in the 

 Ringer's solution contained in the glove when the latter, with 

 the outside of the skin turned outward, is suspended in a N/7 

 NaCl solution. Let us now reverse the conditions and study 

 the behavior of gloves with the inside of the skin turned out- 

 ward, when filled with N/7 NaCl and suspended, respectively, 

 in Ringer minus K and in Ringer. As in the other experi- 

 ments, the Ringer's solution used was approximately isotonic 

 with frog's blood. 



Experiment 556 (fig. 23) 



Pour experiments of this character were made and, in three 

 instances, the behavior observed was essentially similar to 

 that of the gloves graphically represented in figure 23. (For 

 type of experiment see diagrams L and M in fig. 23.) The 

 essential feature to be noted is that after an equilibrium had 

 been established, the glove suspended in Ringer in which K 

 was present (M) began to lose weight, on account of a trans- 

 fer of water from the glove through the skin outward that 

 is, in the normal direction taken by the transfer when the 

 inside of the skin is turned outward. 



VIII. ON A POSSIBLE EXPLANATION OF THE CONTINUOUS FLOW OF 

 LYMPH BETWEEN THE CAPILLARIES OF THE BLOOD-VASCULAR 

 SYSTEM AND THOSE OF THE BLINDLY-ENDING LYMPHATICS 



The results of the experiments detailed in the preceding 

 pages lead us to the conclusion that when the living frog 

 remains in water, three sets of factors may be involved in the 

 transportation of fluid through the skin into the subcutaneous 

 lymph sinuses. These factors constitute, 1) the varying 

 hydration capacity of the tissue colloids by which the skin 

 itself may absorb or lose water; 2) osmotic pressure, and, 

 3) a force, electrical osmosis, which acts independently of, 

 and simultaneously with osmotic pressure, in driving water 

 through the skin into the subcutaneous lymph sinuses. 



