42 CHARLES P. W. MCCLUEE 



Experiment 429 (fig. 16) 



The right and the left skin gloves of the same frog (R. 

 pipiens), with the outside of the skin turned outward, were 

 filled, respectively, with Ringer and Ringer minus K and were 

 then suspended in Ringer's solution. (As in fig. 15, diagrams 

 A and B indicate the type of experiment.) The behavior of 

 the gloves showed that in neither case was there a continuous 

 transfer of fluid through the skin during the first eight hours ' 

 suspension in Ringer. From the eighth hour on, however, water 

 began to diffuse through the skin into the gloves, and a 

 gradual gain in weight of the gloves followed. At the end of 

 twenty-three hours' suspension, the weight of the glove con- 

 taining Ringer in which K was present (A, fig. 16) was 

 greater than that of the glove in which K was absent (B, fig. 

 16). Why, under the conditions of the experiment, water 

 should not be transported through the skin at the time of its 

 removal from the body, but should be later on, has not up to the 

 present time been determined. Such is the case, however, and 

 it is evident that the recognition of this fact explains the 

 aberrant behavior of the gloves in question. 



The writer made sixteen preliminary experiments of the 

 type illustrated by figures 15 and 16, in all of which the 

 results were essentially uniform, in the sense that they con- 

 formed with those of. the experiments mentioned by showing 

 that the ions of K influence the velocity of diffusion. 



We may now proceed to the consideration of a series of 

 experiments based upon those above mentioned, in which the 

 conditions have been still further modified. 



Experiment 567 (fig. 17) 



Two skin gloves removed from the hind legs of the same 

 frog (R. pipiens), with the outside of the skin turned outward, 

 were filled, one with Ringer and the other with Ringer minus 

 K, and were then suspended, respectively, in Ringer minus K 

 and in Ringer. (See diagrams C and D in fig. 17 for type of 

 experiment.) Four experiments of this character were made, 



