HI.] VESSELS, ETC., USED IN CULTIVATIONS. 31 



cultivation in a flask or test-tube, or for inoculating material 

 contained in a test-tube or flask, are thus made : while one 

 hand holds the bulb of the pipette, the other holds one end, 

 and putting at some distance from this end the tube into an 



FIG. 7. STEAMER FOR STERILISING CULTURE MATERIAL CONTAINED IN 



TEST-TUBES. 



i. Wire-net to hold the test-tubes ; 2. Tin vessel ; 3. Wire diaphragm to hold i ; 

 underneath it is water ; 4. Lid; 5. Gas-burner. 



ordinary flame and quickly drawing it out, a point of extreme 

 fineness can be made. The same is done with the other end 

 Such a pipette can be considered as practically closed at both 

 ends. 



