vii.] MICROCOCCUS. 79 



violet with a saturated solution of methyl violet. Their 

 diameter is very small, only about 0-0005 mm. The same 

 micrococci were noticed by Pohl-Pincus in the throat-dis- 

 charge. 1 See Bizzozero's statements mentioned on p. 61. 



8. In cattle plague, also called rinderpest, micrococci 

 have been found in the lymphatic glands by Klebs (1872) 

 and by Semmer in the blood and lymphatic glands (1874 and 

 1881). In conjunction with Archangelski, 2 Semmer 

 cultivated the micrococci obtained from the lymphatic glands 

 of a sheep dead of inoculated rinderpest, in beef broth, in 

 meat-extract solution, and in mixture of broth, peptone, and 

 gelatine at 37 to 39 C. The micrococci grew very copiously 

 as zooglcea and in chains. With these micrococci (of a first 

 transfer or cultivation) a calf was inoculated, and died after 

 seven days from rinderpest. The cultures when transferred 

 lose gradually their virulence from one generation to the 

 next, but animals (sheep) inoculated with these are protected 

 against further virulent disease. Further, cultures exposed 

 for an hour to a temperature of 46 or 47 C. become greatly 

 attenuated in their action, and sheep inoculated with virus 

 thus attenuated are protected against virulent material. 

 Temperatures of - 10 to - 20 C. annihilate the activity of 

 rinderpest organisms. The specific nature of these micro- 

 cocci of rinderpest cannot, however, be considered at all 

 established as in the case of those mentioned above, e.g. 

 the micrococci of erysipelas. 



9. In puerperal fever micrococci have been found in the 

 form of zooglcea by Heiberg, 3 in all affected organs endo- 

 cardium, lung, spleen, cornea, in a case of panophthalmitis 



1 Seen already by McKendrick, Brit. Med. Journ. 1872. 



2 Centralb.f. d. med. Wiss. No. 18, 1883. 3 Leipzig, 1873. 



