XL] 



BACILLUS : PATHOGENIC FORMS. 



129 



of the nasal mucous membrane and internal organs, such as 

 the lung, spleen, and liver, of horses dead or dying from 

 glanders. These bacilli are very minute, being of about the 

 size of tubercle-bacilli (see below), and are brought out by 

 staining the tissues with a concentrated watery solution of 

 methylene-blue, and after this washing with very dilute acetic 



FIG- 61. FROM A SECTION THROUGH THE CARTILAGE OF RABBIT'S EAR IN WHICH 

 ULCERATION HAD BEEN PRODUCED BY INOCULATION WITH NECROSED MATTER 

 OF CALF'S TONGUE. 



1. Cartilage capsules. 



2. Bundles of good bacilli. 



3. Bundles of degenerating bacilli. 

 Magnifying power 700. (Stained with magenta.) 



acid. They succeeded in artificially cultivating the bacilli 

 in the incubator at 38 C, on solid sterilised serum of horses' 

 and sheep's blood, using for the purpose particles (see below 

 under " Tubercle-bacilli ") of nodules of the lung and spleen 

 of a horse dead of glanders. After two days (i.e. on the 



