SPAWNING HABITS OF CH/ETOPLEURA APICULATA.. 25! 



XII. 



Experiment 12, August /-J. 



100 Chitons collected Monday, August i. 



ist night, placed in three dishes on laboratory table; three 

 females spawned very lightly before 10 P.M. about one 

 fifth maximum or less; none after 10 P.M. 



2d night, two dishes placed on table ; one left in running water ; 

 about 75 specimens in all, some having been lost; four 

 females spawned about 25 eggs each by 10 P.M. 



3d night, no eggs. 



Remark. Spawning greatly reduced and all but suppressed. 

 Spawning this year much lighter than last year. 



XIII. 



Experiment 13, August 5-6. 



40 Chitons collected Friday, August 5. 



ist night, placed in two dishes at / P.M.; no eggs or only 

 one half dozen eggs from one female; one male shed 

 spermatozoa before 10 P.M. 



2d night, four females spawned about two thirds maximum 

 and a fifth one half maximum ; impression of fair spawn- 

 ing activity; good for this year; all done before n P.M. 

 3d night, about 25 eggs from each of three females ; practically 



none; done before n P.M. 

 * New moon, August 3. 



XIV. 



Experiment 14, August p-/o. 



60 Chitons collected Tuesday, August 9, but not brought to labo- 

 ratory until next day. 



2d night, two females spawned about one fourth maxi- 

 mum. 



XV. 



Experiment 15, August 10-12. 



25 Chitons collected August 10 Wednesday, 

 ist night, none spawned. 



