RETINAL REFLEXES OF NARCOTIZED ANIMALS. 227 



The animals ceased to respond after six minutes, were transferred 

 to fresh sea-water, but did not recover. A large blennid, appar- 

 ently completely recovered from treatment with camphor, immersed 

 in this solution, gave almost immediately a definite response to 

 "darkness," but none to "light." It ceased to respond after five 

 minutes. 



Camphor (resublimed, i: 16000). One large specimen, about 

 five inches long, gave a definite " light " and a more marked " dark- 

 ness " response after 10 minutes' immersion. These responses 

 consisted of a twitch of the tail and sometimes of the whole body. 

 Tapping the vessel containing the animal induced a similar re- 

 sponse. That to "light" ceased first. After 15 minutes' immer- 

 sion the animal appeared completely unconscious. It was trans- 

 ferred to fresh water and recovered. It gave no definite responses 

 during recovery. A still larger specimen immersed in this solution 

 gave very definite responses within three minutes, those for " dark- 

 ness " being much more marked. The reflex gradually disap- 

 peared. After 13 minutes breathing had stopped, but the "dark- 

 ness " response could still be elicited. After 20 minutes no re- 

 sponses could be obtained to any stimulus. The animal was re- 

 turned to fresh sea-water and recovered. Two small specimens 

 which had been kept several hours in the laboratory were nar- 

 cotized in about 10 minutes. No illumination responses were seen. 



(C) Siphdstoma griscolineatum. A species of pipe-fish about 

 six inches long caught amongst eel-grass at low tide. 



Benzene (i :3OOo). Two animals gave a very positive "dark- 

 ness " response after eight minutes' immersion. That to " light " 

 was less. The response was indicated by a spasmodic jerk. It 

 could be elicited during an additional 15 minutes. Both animals 

 gave marked responses even after tap and tactile stimuli had ceased 

 to produce much reaction, and after breathing had stopped. 



Phenol (1:4000). A specimen, previously treated with cam- 

 phor, and apparently completely recovered, gave, after 10 minutes' 

 immersion, positive "light" responses and more marked "dark- 

 ness" responses. A fresh specimen immersed in a (1:5000) 

 solution gave slight responses after 10 minutes' immersion, which 

 were more distinct for " darkness." 



Camphor ('resublimed, i : 16000). A specimen gave a response 



