242 B. H. GRAVE. 



III. 



June 27-30 : 



15 Chitons collected June 27. 



ist night, four females laid eggs; spermatozoa abundant. 

 2d night, two or three females spawned lightly. 

 3d night, two or three females spawned lightly. 



IV. 

 June 28-30 : 



100 Chitons collected June 28. 

 ist night, no eggs. 

 2d night, great quantities of eggs from 12 to 15 females; 



spermatozoa abundant; eggs laid 9-12 P.M. 

 3d night, two females spawned in quantity; a few eggs 



from several others. 

 4th night, two specimens spawned. 

 5th night, a few still spawned. 



Note. Nearly all spawned second night. Spawning is nearly 

 always on ist or 2d night after collecting. Spermatozoa appear 

 first in every case observed. 



V. 



* Full moon, July i. 

 July 2-3 : 



20 Chitons collected July 2. 



ist night, two spawned, one of these about 10 P.M. 

 2d night, two spawned ; spermatozoa shed in abundance 

 as soon as animals were placed in quiet water. 



VI. 



July 3-4 : 



158 Chitons collected July 3. Placed 25 per dish, 

 ist night, two spawned. 



2d night, about 20 females spawned, and about 30 or 

 more males shed spermatozoa. (This group con- 

 tinued to spawn freely for five nights.) 



