SPAWNING HABITS OF CH/ETOPLEURA APICULATA. 247 



ist night, none spawned in either case; one male was 

 shedding spermatozoa at 7.30 A.M. Sunday morning. 



2d night, of those placed in running water one spawned 

 one fourth maximum ; of those placed on laboratory 

 table two spawned lightly ; several males shed sper- 

 matozoa in quiet water (abundant from 8 to n 

 P.M.) ; very little in running water. 



3d night, those placed on laboratory table spawned about 

 25 eggs; those placed in running water spawned 12 

 eggs ; one specimen spawned in each case ; males 

 shed spermatozoa in considerable quantity in quiet 

 water. 



Remark. Very light spawning from this group. 



* New moon, July 5. 



IV. 



Experiment 4, July 5-7. 



40 Chitons collected July 5. Placed in running water promptly, 

 but not set for experiment night of July 5. 



2d night, set in two lots, one in running water and one on 

 laboratory table in quiet water ; twenty good speci- 

 mens each; of those on laboratory table one spawned 

 100 eggs more or less ; of those in running water two 

 spawned not to exceed 100 eggs each, between 9-11 

 P.M. 



3d night, set as above ; none spawned during the night, 

 males nor females. 



Remark. Almost no spawning from this group. 



V. 



Experiment $, July 7-9. 



80 Chitons collected in afternoon, July 7. Divided into two lots, 

 40 each. Medium size to large specimens. Good lot. One 

 group placed in running water and one in quiet water on 

 laboratory table, as usual, about 7 P.M. 



ist night, none spawned in either dish, males nor females. 



