254 B - H - GRAVE - 



XXI. 



Experiment 21, August 26-28. 



Chitons collected from both Nobska Light and Vineyard Haven, 

 August 26. About 100 specimens, 

 ist night, no eggs. 

 2d night, no eggs up to 10 P.M. ; one female spawned 20 



eggs after 10 P.M. 

 3d night, one female spawned 100 eggs. 



Remark. Practically no spawning. Many specimens used. 

 Season rather late. Spawning sporadic. 



XXII. 

 Experiment 22, August 29-30. 



60 Chitons collected August 29. 

 ist night, no eggs. 

 2d night, six females spawned very lightly one fifth to one 



tenth maximum ; only a few eggs each ; several males 



shed spermatozoa. 



Remark. Represents a distinct increase over preceding nights. 

 None after 10.30 P.M. Spawning per individual pretty light, 

 however. 



XXIII. 



Experiment 23, August ^i-September i. 



120 Chitons collected August 31. Placed in three dishes. 



ist night, no eggs. 



2d night, nine females spawned; three one half maximum, 

 others one fourth to one fifth maximum ; very consider- 

 able spawning and much spermatozoa in one dish; two 

 dishes with little spermatozoa. 



3d night, no eggs. 



Remark. Good spawning activity for time of season. 

 * New moon, September i. 



