150 



JAMES ERNEST KINDRED. 



The activity of the leucocyte was then observed in a hanging 

 drop, but typical clot formation did not occur except where the 

 leucocytes came in contact with the glass. If the leucocytes 

 in this drop were isolated from each other they retained their 

 membranous projections, but if they came into contact with 



FIG. 5. Same group of cells as in Figs. 3 and 4. Clot fully formed. Drawn 

 30 minutes later than Fig. 4. Semi-diagrammatic. X 1,000. c., amibocytes 

 with colorless globules; 1., leucocyte; r., amibocyte with red globules; yl., amibo- 

 cyte with yellow globules. 



each other they tended to stick together and form a mass to 

 which the other amibocytes adhered. A meshwork such as 

 observed under the conditions of evaporation did not appear. 

 I was unable however to observe whether or not the leucocytes 

 formed true plasmodia or only aggregate plasmodia. If their 

 reaction to each other in forming these masses is similar to the 

 manner in which they form the meshwork of the clot then they 

 must certainly retain their individuality and come into contact 

 without cytoplasmic fusion. 



An attempt was made to find out the role the leucocyte played 

 in the regeneration of resected parts. In the case of a perforation 

 or a slit in the peristomial membrane, the leucocytes form a clot 

 along the margins of the wound. This clot gradually extends 

 across the opening and eventually closes it. If a piece of the 

 test is removed, the leucocytes form a clot around the margin of 

 the perforation and after a longer or shorter period depending 

 upon the size of the piece cut out, this clot closes it. The 



