GLYCOGEN IN CELLS OF EMBRYOS. 247 



the presence of iodine. The cytoplasm was not characterized 

 by any structure indicative of this substance, but appeared the 

 same in all of the cells. The processes of the mesenchyme cells 

 had the peculiar lighter areas which in some cases became port- 

 wine colored and in others remained pale yellow. 



SUMMARY. 



The cells of Fundulus heteroclitus grown in tissue cultures 

 contain some substance which behaves in the same manner as 

 does glycogen in the presence of iodine. It is possible that this 

 substance may be glycogen. 



LITERATURE CITED. 

 Bernard, C. 



'59 De la matiere glycogene considered comme condition de developpement 



de certain tissus, etc. Jour. Physiol., II., p. 26. 

 Best, F. 

 '06 Ueber Karminfarbung des Glykogens und der Kerne. Zeitschr. f. wiss. 



Mikr., XXIII., 319. 

 Dederer, P. H. 



'21 The behavior of cells in tissue cultures of Fundulus heleroclitus . 

 Gage, S. H. 



'17 Glycogen in the nervous system of vertebrates. Jour. Comp. Neur., 



XXVII., 451. 

 Lewis, M. R. 



'17 Sea water as a medium for tissue cultures. Anat. Rec., X., 287. 

 Lewis, M. R. 

 '18 The formation of the fat droplets in the cells of tissue cultures. Science, 



N. S., XLVIIL, 398. 

 Lewis, W. H., and M. R. Lewis. 



'15 Mitochondria (and other cytoplasmic structures) in tissue cultures. Amer. 

 Jour. Anat., XVII., 339. 



