148 JAMES ERNEST KINDRED. 







In both of these experiments the amibocytes other than 

 phagocytes did not act as phagocytes. This may be due to the 

 fact that, if they are, as assumed, derived from leucocytes in the 

 first place by the inclusion of reserve food material or absorption 

 of nitrogenous waste, the material so added, inhibits any phago- 

 cytic activity. 



II. REACTIONS TO VITAL STAINS. 



No success was met with in the attempt to stain the leucocytes 

 by the injection of weak intravitam stains. Aqueous solutions 

 of methyl blue, methyl green, Janus green, and trypan blue 

 were introduced into the perivisceral cavity, but no effect on 

 the formed elements was noted. 1 A 1:500 methyl blue-sodium 

 chloride solution had no effect upon the leucocytes, but faintly 

 stained the globules in the red amibocytes ("globules amceboide 

 brun d'acajou" of Erdle). 



One c.c. of .01 per cent, neutral red in sea-water was injected 

 into the perivisceral fluid of several individuals and after twenty- 

 four hours all of the leucocytes in several drops of the perivisceral 

 fluid examined contained yellow globules. This indicated that 

 the hyaline globules apparent in the leucocyte under normal 

 conditions have an alkaline content. 



III. CLOTTING. 



A drop of the perivisceral fluid of Arbacia, freshly drawn, 

 when placed on a cover slip immediately shows signs of aggluti- 

 nation. The large pigmented and colorless amibocytes appear 

 in long strings held together by the clumping of the leucocytes, 

 although in many cases the leucocytes had not lost their mem- 

 branous processes. A drop of the perivisceral fluid was placed 

 on a glass slide and observed continually for two hours. Sketches 

 of the changes which were taking place in the shape of the cells 

 in a restricted area were made at intervals of thirty minutes in 

 order to show the role played by the leucocytes in the formation 

 of the clot. When first observed there were four leucocytes 

 in the field, each possessing the typical membranous projec- 

 tions (Fig. 3). As the fluid evaporated during the course of 

 an hour these leucocytes came into contact with the slide. 



