170 



CARL RICHARD MOORE. 



TABLE IV. 



transparent jelly except the control lot. In C, eggs to which 

 sperm had been added, only 85 per cent, had been fertilized. 

 There were then 15 per cent, of the eggs still capable of producing 

 fertilizin and we see that a ten-second reaction was given after 

 the eighth washing. 3 Neither is this due to the presence of 

 jelly for this had been dissolved by means of a short butyric acid 

 exposure. In many of the experiments the jelly was destroyed 

 by shaking the eggs a few times in a test tube before the series of 

 washings had begun; the jelly is very easily removed in this way 

 and in all cases where an appreciable number of eggs were not 

 fertilized the agglutination reaction could be obtained. 4 



1 Eggs i part, sea water, 4 parts. 



2 Cleavage was so irregular and cytolysis beginning in so many, that the count 

 is probably far from correct. Only a very small per cent, ever reached the larval 

 stage where even a slight amount of motion was discernible. 



3 In many lots of eggs especially resistent to sperm practically as high an ag- 

 glutination action was obtained as in eggs easily fertilized. Lack of fertilization 

 is evidently due to physical conditions that prohibit entrance of sperm. When 

 100 per cent, of fertilizations are obtained no agglutination is found, provided 

 that the time limit of fertilization is complete and also that the eggs have been 

 deprived of their jelly that is saturated with fertilizin liberated from the egg. 



4 It must be emphasized that one should use only freshly prepared sperm sus- 



