172 CARL RICHARD MOORE. 



Lillie ('14) has already given pi oof of this as is shown in his 

 Table VII, page 560. The present results, however, confirm his 

 assertions that wherever fertilizin is absent the fertilization 

 capacity is also entirely lacking. A typical experiment, one of a 

 large number performed, will give the results of the whole series 

 of conditions in which the eggs would not respond to the influence 



of sperm. 



Experiment 65. August 26, 1915. 



9:00 A.M., eggs collected, washed and divided into three lots 

 A,B, and C. 



A . Exposed to butyric acid for 20 minutes and returned to alka- 



line sea water to neutralize acid. 



B. Exposed to sea water 35 C. for 10 minutes, shaken very 



gently to free from surrounding jelly. 



C. Exposed to butyric acid for 20 seconds, returned to alkaline 



sea water to stop action of acid. 92 per cent, of eggs 

 produced membranes. 



Eggs collected into graduated cylinders and volume of egg 

 noted. 



A, i.i c.c.; B, i.o c.c.; C, 2.0 c.c. 



10:20 began a series of washings. Results given in Table V. 



Thus in each of the three cases no cleavages occurred and no 

 fertilizin is being produced. The butyric acid exposure of 20 

 minutes in this experiment is much longer than necessary to 

 prevent fertilization as was learned later. But in experiments 

 where a much shorter exposure was employed, and a small 

 percentage of cleavages were present, fertilizin was present. 



In all instances then in which eggs have been artificially altered 

 in their fertilization capacity we have seen the absolute corre- 

 spondence of the presence or absence of fertilizin. In the above 

 instances we may use the agglutinin reaction as a test for the 

 capacity of fertilization. The writer does not wish to be under- 

 stood as maintaining that no other processes are involved in 

 these conditions but in whatever condition we have encountered 

 the egg, if it was found to be actively secreting fertilizin, fertiliza- 

 tion has been possible at least to some extent; wherever fertilizin 

 was absent we were never able to obtain fertilization. The 

 correlation is a very close one and one appearing to be of great 

 significance. 



