TWENTY MONTHS OF STARVATION IN AMIA CALVA. 459 



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FIG. i. Microphotograph of body muscle of Amia calva, showing normal and 

 partly broken down muscle cells. Stained with Wright's blood stain. Magni- 

 fication 600 X. 



In Fig. 2, a second microphotograph, are shown some normal 

 muscle fibers, others partly broken down and one entirely empty. 

 In the empty fiber, the muscle nuclei are still arranged along the 

 cell wall of the muscle. One of these nuclei has divided. Three 

 red blood corpuscles appear near this divided nucleus and furnish 

 a good comparison. The appearance of the blood corpuscles as 

 photographed indicates that the cells are well fixed. 



These two microphotographs clearly indicate that the striae, 

 then the sarcoplasm and finally the nuclei is the order in which 

 the several parts of the muscle cell break down in Amia during 

 starvation. 



Fig. 3 is a microphotograph of a dividing muscle nucleus and 

 the method is certainly amitotical. These nuclei become sepa- 

 rated from the cell wall and gradually fragment. Several smaller 

 pieces are seen in this figure. 



A variety of stains was tried on the material fixed and sectioned 

 but the results were unsatisfactory. The muscle sarcoplasm 

 and nuclei stained very faintly. In one slide stained with 



