214 WM - A - KEENER AND J. G. EDWARDS. 



cysten der Cryptomonaden zu handeln." There are also asso- 

 ciated with the pharynx two flagella of equal length. Eyferth 

 ('oo) says that these arise from the dorsal wall of the pharynx; 

 Jennings ('06, Fig. 72) in his figure does not commit himself 

 as to the place of origin of the flagella. Calkins ('01, Fig. 10, B) 

 and Pascher ('13, Fig. 171) show one flagellum arising from the 

 dorsal and the other from the ventral side of the pharynx. A 

 contractile vacuole is within the dorsal anterior extremity of 

 the body. The general cytoplasm is not alveolar, though it 

 usually presents such appearance, because of the presence of 

 colorless, highly refractive spheroidal bodies. It is important to 

 "note that these bodies vary in number, and in so far as they 

 decrease in number the apparent alveolar condition of the cyto- 

 plasm becomes less evident. We have tested these with the 

 iodine starch test and got a negative reaction the bodies 

 staining brown. These bodies have the same general optical 

 features as the paramylum grains of Euglena and have been 

 described for Chilomonas as paramylum grains; they are, there- 

 fore, to be looked upon as assimilation products. These assimila- 

 tion products are more or less directly related to certain deeply 

 staining (nuclear stains) rounded bodies, which lie about them. 



Most systematic and experimental workers, when describing 

 Chilomonas paramozcium have paid little or no attention to these 

 chromatic bodies. For example, Eyferth ('oo), Pascher ('13) 

 and Jennings ('06) neither describe nor depict them. These 

 same authors, however, describe a centralized well-defined 

 nucleus lying behind the middle of the body. 



Calkins ('99) recognized no nucleus in this rounded, nuclear- 

 like structure, which lay behind the middle of the body; but he 

 looked upon the deeply staining bodies which lie by the para- 

 mylum grains as a distributed nucleus, and thus brought Chilo- 

 monas paramcecium in line with a series of animals bearing 

 distributed nuclei or permanently granular nuclei. "Forms 

 with this permanently granular chromatin, again, are found in 

 two conditions. In one type the granules are scattered through- 

 out the entire cell, and are never confined by a nuclear membrane 

 (so-called 'distributed' or 'diffused' nuclei). In nuclei of the 

 other type the granules are confined in a definite, more or less 



