(ENOCYTES OF PLATYPHVLAX DESIGNATE. 



he found that the methylene blue had passed into the lumen 

 of the Malpi^hian tubes in sufficient quamitie- to odor them; 

 the oenocytes at the end of an hour and a half had lost their 

 color. 



Following out this line of inve-ti-ation. a do/en or more -peei- 

 men- of Platyphylax Jcsi'^nalns were injected with an aqueous 

 solution of methylene lilne and the pan- di ccied and remo\ t d 

 to a jJ v <iTine mount. It v. a found that immediately afu-r 

 injection the oen< and the spinning-glands, SO prominent in 



tlii- form, had both taken up the -tain the tenoeyte- t< ( a much 

 1. - marki-d degree than the latter. In onlv OIK- in-tance. \\a- 

 it found that the Malpi-Jiian tube- had taken up the methvleiie 



blue, and in this case the color was seen in but a -in-le tube. The 

 iii' ;o,k ii]> the -tain more in the micK-n- than in the , \ tO- 



|'la-in. th- latter. houe\er, beini.; distinctly tinned. The Iar\.e 

 killed a halt hour alter injeition shou ed the tEnOCytCS more 

 decjiK : lined, but no . ion was ob-er\ ed in t he Malpii;hian 



tllbe-. l.alA.e killed niie hour .ll'tlT illieclioii -ho\vcd that the 



Mal|.ichian tube- had be^un to take up the blue color, but that 

 the 01 and spinning-glands at tin- time \\ere becoming le-- 



inten-e in ilu-ir i o[ ( ,rat ion. The-r n--ult- \\ould lead one to 

 rather dilleieiit ( mu lu-ion- than tho-i- reached b\ llolm^nn. 

 It \\oulil be interred from the -imilarity of the reaction ot the 

 <i-ii' and Spinning-glands as detailed abo\e, that the former 



h ot a d tteretu nature and inien^itx to that 

 ot the vpiiiiiiiie.-ij.md-. 



I hi- \ie\\ i- further !u-neil b\ injection experiment ^ 



with sulphindigotate of sodium, l-'iirth 190 l)iee\' 



|oii-i In- I'.edt lining der M a! i <\-^ hi'-chen ^chlauche oftenbart sil h 

 am h in un/\\eidt: \\'ei-e dun-h ihr \ermo-cn ,lir AII-- 



-cheidiin^ VOn 1 arb-totfen /u bewerkstelligen." >chimller I - 

 found b\ injection ot a -olutioii ot sulphindigotate oi -odium into 

 the bod\ cavity ot < '. yllotnlf'.i that the Malpii;hian tube- 

 excr< I'd tlu- -ub-taiiie \\ithin twenty-four hour-. 



An ai|iicoii- -olution ot sulphindigotate of -odium \\a- injected 

 into the abdomen of a do/en or more ]ar\.e of Flnfyphylax ./r>.';-- 

 tiatits 15 2O mm. in length, the in-ertion of the hypodermic -vrinue 

 1'einv. \ entral and 1 1 but tin- -olution ( \i--ndin.u thoroughly 



