DIFFERENTIATION OF THE HTMAX CELLS OF 



SERTOLI. 



I II' (MAS II. M"\ K.' 'MHKY. JR. 



-ITY OF I. VAN! A. 



This study is based on the examination .f the testis "I a 



ro aliout 40 year- of ,i-c. piv^TM-d in Xenker's fluid \\hile 

 Mill \\arm after hi- e\eeiitioi). The fixation \\.i- not as excellent 

 .1- niiijit In- desired, cytop'.a-mic detail- ln-iu- not always pre- 

 served, 1'Ut the preservation "f tin- nuclei \\a- mi tin- \\ln>!e MTV 



d, and of -pindle fiii'ii'' Mcni. A considerable \ariety ot 



>taiuiii'j IIH thods were cinploytd, of \\hieh the nio-t Iruitful 

 |ii.i\t-il id In- 1 Icidnihain'- iron li.rmaio\\ line, \\ith \ arion> 



'i- of r\tr.i< lion, follout-d |i\- .ili'oholi.- eosin. 1'araltiin- 



ions were made of 5/1 and Sju. 



I op tlie '^ift of tlli'- malt-rial I am indrlit d to tin- kindiic-- of 



I >i . . \ddi-on, of the I 'ni\ i-r-ii > of Pennsylvania. 



i. GENERAL < M M.IM >i mi PROCESS. 



'I'hf tf\t diagram exhiliii- tin- i hi' I iv-n!'- oliiaint-d. Tin- 



antepenultimate spermatogonia t-oniain eaeh a rMi (R.) \\itliin 



ihc ( \ lop!.i-m. This doe- not di\ idt- in inilo^i-. i \\^ i|iifiitly 



iu-t half of their daughter cells, the penultimate spermatogonia, 



foiiit- to i-oiiiain each a rod. \\hilt- half of iln-in laek it. In iln- 

 di\i-:on of thf-c pcnultiniate spermatogonia thf rod ddi- not 

 di\idt- Inn lu-foiDf- di-ti-iliutfd to oiu- nuartfr of the ultiinatf 

 -|i- i inaloLoiiia. Each of ever} llm-f ultimate -ptTmato-onia 



product-^ 1>\ di\ ision two primarv spermatocytes, and ihf-t- (<!!- 



\\ h ifh I n -Ion- to the true .germinal c\ clt- laek the rod f mi rely. But 



ii four ih ultimate spermatogonium presen es the rod. and this 



cell without further division enlarges and becomes a cell of Sertoli. 



In t hi- cell of S'l'ioli a ]>rimar\ n>d let (r. j) buds oi'f from the rod : 

 then the rod disappears, while the prim ili-t di\idf> inio 



t\\o -econd.ii \ rodlei- r.2 ind the latter persist in the Sertoli cell 



throughout it- hi-lory. 



367 



