50 i.ARY X. CALKIN-. 



vacuolc. Its -nuill size \\a> due to the prox.mity of the cutting 

 plane to that of the normal division plane of the cell. It died 

 on the second day after cutting (Plate I., Fig. 8, a, b, c, d}. 



(c) Division of the Ahaomni! Cell. In one experiment of this 

 series (no. 54) the abnormal cell, resulting from the division of 

 the origin. il cut fragment, divided three times. The vegetative 

 cell was cut as shown in Fig. 7, a. After 24 hours the fragment 

 was lively and complete as far as the plane of the cut which was 

 sharply marked (Fig. 7, b). After 48 hours it had grown much 

 larger and was particularly broad at the anterior or truncate 

 end. After <>6 hours it had divided into an abnormal truncate 

 cell (7, c, and 7, d) and a normal cell (7, ?) On the sixth day 

 this abnormal cell had grown in size but retained the original 

 truncate anterior end (~,f) while the normal cell had divided to 

 form two normal cells of which one was cut (see Table III., no. 

 38) and the other lost in cutting. On the seventh and eighth 

 days the abnormal cell had grown very large again and on the 

 ninth day divided to form two small dissimilar cells (7, g, //), 

 each, however, with only one vacuole. On the twelfth day each 

 of these divided once more giving rise to four small abnormal 

 cells (7, /', j, "k, /), one of these (/) dying on the same day, two of 

 the others (7, m, ) dying on the seventeenth day and the fourth 

 (0) on the eighteenth day. 



This experiment resulted in two races of cells, one of which 

 (from the posterior end) were normal, the other abnormal. The 

 part lost was never regenerated and the abnormality was finally 

 transmitted to the posterior end of the original fragment. 



(d) Monster Forms. These will be considered together with 

 those of Table III. (see page 53). 



(e) The three cases of regeneration were nos. 4, 11 and 14. 

 ( M these no. 4 was cut during division of a vegetal ive cell. Within 

 half an hour after the operation the division was completed, one 

 perfect cell and a small imperfect cell resulting. In 24 hours 

 both were living and both were of normal -hape but one was 

 much smaller than the other. At the end of 48 hours the small 

 one had ^io\\ n t<> \\ normal cell both in size and shape, but on the 

 third day it was dead. 



No. II was al-n a dividing cell when cut through zone 2. ( )n 



