EFFECTS PRODUCED BY CUTTING PARAMECIUM CELLS. 5! 



the following day the larger fragment appeared entirely normal 

 save for an accumulation of crystals at the anterior end <J ; ig. 10, 

 Her cell wa- truncate. On the second day l>otli were 

 normal. 



No. 14 \\a- a large vegetative cell which had been treated with 

 neutral red prior to cutting. The -mailer fragment, contrary to 

 the ii-ual hi-tory, remained alive for -i\ hour- hut was dead the 

 i\. The larger fragment did not regenerate within 4^ hours. 

 The \\.iti h glass i 'i Main ing the fragment was not examined again 

 for a period of \<-\ en days when it was found to,, .main 12 1. 

 giant lonn- .md mall form- normal save fr -i 



.1 ;////;> /v '.II. 



'Ill-- (i-ll- cui in /on,- ill. gave a higher percentage ot li\ing 

 fragments than tho-e cut in /one 1 1. 4*> per cent. continued to 

 li\- afler 24 horn- as against 42. <> p< -r cent.; \\hile 36.6 pet rent. 

 loiitiinied to H\. 48 hours as again-t Jo.o per rent. The 



pencil iinni-ler> in both was .ibout the same. \i/.. 



[2.2 per cent, and i.vJ per rent . but there \\a- not ,i -inglr case 

 ot regeneration. ( M those that li\ ed Inn did not regenerate, - 

 .II- ju-t after conjugation; others - \\ere < ..niu 



\\heii cut. \\hile the renuiindiT \\ere laro- \<^,tati\e torm-. 

 Tin- reai limi- tor tin- most part were -imilar to tho . II- cut 



in zone 1 1., the con . forms, or tho-r ju-t mu of (-onju^ai ion. 



gi\ini4 the oiil\ n,.\cl n -nits. Tin i. 2, ;. }. s and 



I ,: ; ', III. Ill nos. I. ^ and 4 the cell- u i -n infant - 



\\hen cut, the fiamiicin- being kilK'd alter <x> hour- foi tin- 

 determination of tin- nucli-ar londitioii. Fig. u -hn\\- the 

 -tructure of nos. I. .; and 4 .it thi- perio<l. the ch.n acteri-t ic 

 fia^meiited nucleii- -ho\\in- .1 lerminal ol i "iiju^at imi. 



It i- po il.le that tin-, c.-ll- \\oiild ha\e continued to li\e and 

 to di\ide had the\ had a longer time, lor the\ \\i-n a, the and 

 normal in appearance u hen killed. It was quite Otherwise, ho\\ - 

 ever, \\ilh ci-11- i ut dmiiu coiiiugation nOS. -. ^ and o . In all 

 Cases the fragment- died u it hiu four da \ - \\ it limit .my attempt 

 at regeneration. In one case no. s the cells were -till united 

 but dead alter J-4 hour-: in another no. <) both li\ed for several 

 da\- \\ithoiit regeiieraling or dixiding- in another case no. 21 

 the\ -eparated and li\ed for 4.^ hours \\hen both died. 



