ABNORMAL MITOSES IN SPERMATOGENESIS. 193 



CASE 3. 



The occurrence of divisions with abnormal numbers of both 

 chromosomes and centrioles is of common occurrence in the matu- 

 ration divisions in Hemiptera (see for example Henking, '91), 

 and such divisions have also been described in spermatogonia 

 (Montgomery, '98), and in cells from the connective tissue of 

 the testis (Paulmier, '99). The general features of these divi- 

 sions are well known, and they probably originate from abnormal 

 spermatogonial divisions in which the daughter chromosome 

 groups have failed to produce separate nuclei. In the cases here 

 noted, from Chlorochroa iihlcri ( = - pcrsiniilis) Stal (Family 

 Pentatomida?}, large numbers of cells were affected particularly 

 along one half of one testicular lobe, and to a lesser degree the 

 contiguous portion of an adjacent lobe. In some cysts, as could 

 be told from the unusual size of the nuclei with extra chromo- 

 somes, large numbers of spermatocytes (and spermatids) were 

 affected, while in others all the cells were normal. The causative 

 agent operative in this case would accordingly seem to differ 

 from the more or less accidental sources of abnormal division 

 figures. The chief interest of this case lies in the fact that the 

 material had been prepared to demonstrate the Golgi apparatus, 

 and it was accordingly possible to study the distribution of the 

 dictyosomes (fragments of the Golgi apparatus) in relation to 

 the multipolar spindle. 



Numerous cases of tripolar 1 spindles in the first maturation 

 division were found at both the metaphase (Fig. 2) and late 

 anaphase (Fig. i) stages, of which two are figured. As I have 

 shown in another place (Bowen, '20), the dictyosomes collect (in 

 equal amounts) around the ends of the normal (bipolar) spindle 

 at the beginning of the metaphase, a position which they main- 

 tain during the anaphase, the Golgi material being thus distributed 

 with approximate equality to the daughter cells. I suggested that 

 the centrioles represent the morphological foci of the influences 

 which bring about this equal distribution of the Golgi material. 



i There were also numerous cases in which the centriole number was nor- 

 mal, but with abnormal chromosome numbers, as in Case 2 ; but as these pre- 

 sented no points of special interest, they are not here considered. The 

 distribution of the dictyosomes was in accordance with the expectation for 

 a bipolar spindle. 



