416 E. E. JUST. 



9:00 P.M. No development. Eggs washed in 250 c.c. sea- 

 water. Set aside; on settling, removed to 250 c.c. of sea-water. 



July 20, 8:00 to 9:00 A.M. These eggs show a very small 

 per cent, fine top swimmers. Undeveloped eggs show sperm at- 

 tached. Top swimmers removed. 250 c.c. of fresh sea-water 

 added to remaining eggs. 



10:20 A.M. About i per cent, of eggs in the 2- and 4-cell 

 stages. 



10:25 A.M. Eggs inseminated in 250 c.c. of fresh sea-water. 



ii : 25 A.M. 5 per cent, of eggs in 4-cell stage, 85 per cent, in 

 2-cell stage. Some others show spindles, remainder no change. 

 Eggs tend to lose membranes and to bud. 



4:00 P.M. Small per cent, of eggs in swimming stage. 



6:00 P.M. About 90 per cent, swimming. 



9:00 P.M. Swimmers at the surface. 



The chief point of interest in this experiment is that eggs in- 

 seminated in blood may, on washing, develop normally. If por- 

 tions of such eggs are washed at intervals up to about two hours 

 after insemination, a high per cent, develop perfectly. They lose 

 their power to develop on washing two to three hours after in- 

 semination. This seems to be the most significant point of this 

 report (see beyond, page 421). Eggs inseminated in the presence 

 of blood take in sperm ; such sperm lie within the cortex. All 

 these eggs show sperm firmly attached to the cortex. These eggs 

 have been studied not only while living, but also in sections and 

 in toto mounts properly fixed (Bouin, Lang, Meves, Flamming). 

 In some cases the sections show the egg cortex literally studded 

 with sperm, with other sperm within the egg. The failure of 

 these eggs inseminated in blood to develop is not, therefore, due 

 to failure of sperm attachment or penetration. On washing within 

 about two hours after insemination in the presence of blood the 

 eggs are released from the blood block. The per cent, of eggs that 

 develop depends upon the thoroughness of washing within this 

 two-hour limit as well as the amount of inhibitor present. 



This experiment likewise reveals that twenty-eight hours after 

 their insemination in the presence of blood eggs, after washing, 

 will fertilize with a high per cent, of development. 



