2l8 CHAS. W. HARGITT. 



strong confirmation of Montgomery's views. This point may 

 have further consideration in connection with observations upon 

 the history of the nucleolus. 



Concerning the real nature of these granules there arises the 

 query, are they katabolic products, associated with some vital 

 wastes incident to the cytoplasmic activities of growth, or are 

 they not rather anabolic in character, highly nutritive proteid 

 bodies, analogous to yolk matter and of similar import ? The 

 latter is by far the more probable view, though there are points 

 of difference as compared with the usual formation of such nutri- 

 tive matters. For example, in most hydroid eggs which have 

 come under my observation the development of yolk granules 

 has no appreciable relations to nuclear influence, and seems to be 

 for the most part developed and deposited chiefly at the vegetal 

 pole of the egg ; while in the present case, as has been shown, 

 they seem to arise and develop chiefly in the nuclear area and 

 only at a late period are found at the vegetal pole. However, I 

 am inclined to believe that this is not a serious difficulty. It will 

 be observed that the vegetal pole lies in immediate contact with 

 the spadix of the gonophore, and that the reception of nutritive 

 matter by the growing egg is from this source. Of course, this 

 nutritive matter is in the form of liquid, and so long as the egg 

 is continuously receiving it in this way there is no occasion for 

 further metabolism into the more solid reserve of yolk. The 

 remoter animal pole where such reserve would first be needed 

 begins the anabolic process first, and with the gradual suppression 

 of the nutritive activities of the spadix the process of proteid anab- 

 olism would extend into that area. 



According to Harm ( op. cit., p. 19), the development of this 

 yolk matter in C. squaniata presents some rather sharp contrasts 

 as compared with the above. For example, instead of develop- 

 ing more or less gradually, and spreading from the nuclear region 

 over the surface of the egg, he finds it arising somewhat suddenly 

 and equally throughout the entire egg. " Bald nachdem die 

 Eizellen Glockenkern erreicht haben, beginnt in ihnen die Dotter- 

 bildung, die an alien Stellen zu gleicher Zeit einsetz." He also 

 finds that before the formation of yolk granules the eggs are red- 

 dish in color. " Wahrend die lebende Eizelle vor der Dotterbil- 



