INTERACTIONS OF PROTOPLASMIC MASSES. I - 1 I 



(e) JO Per Cent Hay Infusion Containing 0.025 Per Cent Sodium 



Carbonate. 



By beginning with one per cent, sodium carbonate and each 

 time decreasing the proportion, it was determined that .4. />o/v- 

 pora could be successfully grown in a medium containing 0.025 

 per cent, of this substance. Only one set of experiments was 

 conducted, but the results obtained were very definite. On 

 June 2Qth an individual was taken from clone K and set aside to 

 become the progenitor of a new clone S. The next day it had 

 divided, so one of the daughter-cells resulting from this division 

 was placed in 10 per cent, hay infusion containing 0.025 P er cent, 

 sodium carbonate, while the other was kept in standard medium. 

 The size, activity, and rate of reproduction of the organisms sub- 

 jected to the influences of sodium carbonate (a weak base) seemed 

 to be perfectly normal. Results of the reactions between line s 

 and line s-s.c. are given by means of symbols (See footnote page 

 1 1 6 for meanings). On the second day after the experiments were 

 begun the interactions were H K 4th o o +, 6th line S s.c. per- 

 manently transferred to standard medium, 8th - -, Qth H (-, 

 nth + + , 1 4th - o, 1 9th , 2 ist 23d - -. No further 



observations were made after this date. These results strongly 

 suggest that, if one line of a clone be placed in 10 per cent, hay in- 

 fusion and the other line in a similar medium to which 0.025 P er 

 cent, sodium carbonate has been added, the physiological consti- 

 tutions of the members comprising the two lines will quickly 

 become so differentiated that cross-fusion will not take place 

 between individuals belonging to one line and fragments of speci- 

 mens from the other. Such changes are shown to persist for some 

 time after the causative agent has been removed. 



(/) 10 Per Cent. Hay Infusion Containing 1 Per Cent. 



Alcohol. 



On August 28th specimens were taken from two (loin--, which 

 had been under observation since June 251)1, and \\ith tla-in two 

 sub-clones, J and K, were started. These Mib-rloiu-; v 

 divided into two lines each; one of which w.i^ krpt in M.md.inl 

 medium, while the other was placed in 10 pi-r rmt. h.iy inl'ii-imi 

 plus I per cent, alcohol the latter bun- iir-i;;n.iti-o! MI!>-<IMIH- 



