122 BRUCE D. REYNOLDS. 



J line j-a, and sub-clone K line k-a respectively. The organisms 

 subjected to alcohol seemed to thrive quite well the rate of 

 fission, in both lines being slightly greater than that of those kept 

 in standard medium. It was found, by testing the interactions 

 of related specimens grown in these different environments, that 

 the results were parallel in both cases; yet different from those 

 obtained when substances other than alcohol were used for alter- 

 ing the environmental conditions. These observations were 

 extended over a period of 65 days, representing 66 generations 

 in sub-clone J and 63 in sub-clone K. The results are indicated 

 by symbols (see footnote on page 116 for meanings). For 

 sub-clone J, reactions between line j and line j-a were as 

 follows: on the 3d day + o, 5th + o, '8th - -, loth 



+ +, 14111 - - o , 1 6th -- o, i8th -- o o, 2ist + + , 23d + 

 + +, 29th oo-, 33d + + +, 35th- -, 461)1 --o- 

 63d - -,65th -- o. 



For cub-clone K, the reactions between line k and line k-a were 

 as follows: on the 3d day o +, 5th + o, 7th + , 8th + + + +, 

 9th - -, loth + + +, nth + + +, I4th - -, i6th 



+ + 0, i8th + +, 2ist + + + + +, 2 3 d + +, 28th --oo 

 o, 30th + +, 35th + +, 46th o + +, 52d + +, 63d - -, 

 65th - 



As can be seen by glancing at these data, the interactions were 

 variable and inconsistent. This suggests, perhaps, a fluctuating 

 change of the protoplasm in contradistinction to the uniform 

 conditions found to prevail in other experiments included in this' 

 section. No explanation of this is offered, since time did not per- 

 mit an exhaustive investigation of the subject to be made; but 

 it seems that one would hardly be justified in attributing the 

 cause to a detrimental environment, for, as has already been 

 dointed out, growth was not diminished by the presence ot 

 alcohol. Hegner ('19), found that Arcella dentata would grow 

 and reproduce in a medium containing from 0.25 to I per cent, 

 alcohol. However, the fission rate was retarded and irregulari- 

 ties in the shells of the offsprings were observed. 



