CELL BEHAVIOR IX TISSUE CULTURES. 



HUBERT B. GOODRICH, 

 WESLEYAN UNIVERSITY AND THE MAKINI: BIOLOGICAL LABORATORY. 



This paper deals with certain free wandering cells observed in 

 tissue cultures from the minnow, Fundulns lieteroditus and 

 Fundulus majalis. The types of cells which became free and 

 isolated from the spreading tissue growths were, chromatophores, 

 amoeboid mesenchyme cells and, most abundant of all, certain 

 cells having curious fan shaped projections. These cells proved 

 to be identical with those studied in their association in tissues 

 in cultures by Dr. Dederer ('21). She identified these as mesen- 

 chyme cells which in cultures were the means of attachment of 

 the outgrowing sheets of ectodermal cells to the surface of the 

 coverslip. In the work here presented isolated cells were sought 

 as the best objects for the study of cell behavior. The mode of 

 locomotion and the tactile reactions were more especially studied 

 and for the latter work the Barber microdissection apparatus was 

 utilized. The tissues were cultivated in the sea water medium 

 (M. R. Lewis, '16) using, however, in many cases more dilute 

 solutions. Observations were usually made within two days 

 after planting. The work was done at the Marine Biological 

 Laboratory at Woods Hole, Massachusetts during the summers 

 of 1921 and 1922. I wish to acknowledge my indebtedness to 

 Mrs. D. B. Young for the drawings from the stained preparations 

 and to Mr. S. C. Williams for aid in certain of the observations 

 on the rate of motion of cells. 



The Fan Cells Among cells of this type an abundant form 

 was that for which I came to use the descriptive term "Canoe 

 cells." When first observed these seemed to be elongate spindle- 

 shaped cells such as indicated by many outlines in Fig. 4. I 

 supposed that there were delicate psuedopedia at either end but 

 upon plotting the direction of the motion of these cells I was 

 surprised to find that they were moving steadily at right angles 

 to the long axis. More careful observations upon living and upon 



fixed and stained preparations showed the presence of a delir.it r 



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