INTI-.kA' 1 ION'S OF PROTOPLASMIC MASSES. 



115 



toplasms). This table (Table II.) shows a range of from 18 to 

 27 generations, and from 15 to 32 days, in the time of the first 

 appearance of negative reactions between two lines of a clone, or 

 an average of 22.25 generations and 21.58 days. After two lines 

 had once become negative to each other no further cross-fusions 

 took place between them unless certain modifications were made, 

 the nature of \vhirh will be discussed later. 



TABLE 1 1 . 



TABLE SHOWING DATE AND GENERATION OF FIRST EXHIBITION OF SHATTERING 

 REACTION IN TWELVE CLONES OF ARCELL.C. BOTH LINES OF THESE CLONES 

 WERE EXPOSED TO SIMILAR CONDITIONS. 



He-fore these cultures were discarded the culture medium, in 

 which the organisms were living, was tested for its pi I \.iliu- by 

 means of the Phenolsulphonephthulein indicator. In r\n\ 

 it was found to be neutral; i.e., no detectable change had taken 

 place because of the presence of the org. mi-ins. 



SUMMARY OF SECTION I. 



In summing up the results obtained I'nun tlu-e experiments, 

 the evidence indicates that physiological difleremv- do de\elop 



'Cross-fusion obtained on the i6th day. Cultuir< >lin| l :;h.-r obser- 



vations were made. 



1 Cross-fusion obtained on the xoth day cultun - ill- 

 I'liis set of experiments is given inexu-nsn in T.iMr I 



