INTERACTIONS OF PROTOPLASMIC MASSES. 125 



If the one shattering reaction observed on the 6th day of the 

 >eriment between the two lines of clone Y be disregarded, the 

 above d.it a will show that negative interactions first occurred <n 

 the 141(1 day in clone X, and on the iyth day in clone Y. Taking 

 tin- hi^hi T figure 17 it is 4.58 earlier than the average time re- 

 quired when the environments in the two lines of a clone were 

 similar. However, if we consider the number of generations, 

 a greater difference is shown. On the day the first negative 

 reaction was observed in clone X, line x had produced 15 genera- 

 tions and line x-/ 8, which if added and the result divided by 2 

 yields 11.5 generations, while in clone Y, line y represented 18 

 generations and line y-/ 10, or an average of 14 generations, 

 in, if we take the higher number it gives a decrease of 8.25 

 generations. It seems then, that if two lines of a given clone 

 are subjected to different temperatures, they tend to i!i\< 

 physiologically more rapidly than when kept at the same tem- 

 perature. 



SUMMARY OF KXPKRIMKNTS INVOLVING DIFFERENT KNVIRON- 



MBNTS. 



As previously shown, when two lines arising from daughter- 

 cells are subjected to similar environmental influences the 

 property of fusing with each other's detached fragments of pro- 

 toplasm ceases after approximately 21.58 days and 22.25 genera- 

 tions. By keeping one line in 10 per cent, hay infusion at room 

 trni| u-raturc in front of a window, such intra-clonal different ia- 

 tii'ii could be greatly hastened by altering (in only one resp 

 t In- other, as follows: 



Differonti.it in 

 Factor imposed. Days. I'-rations. 



25 per cent. 1 hay infusion used 8 



i per cent, sucrose added 6 7 



N/io saline added (unfavorable) 



o.oi per cent, acetic acid added (killed niv-mi-in 



0.025 sodium carbonate added 7 7 



i per cent, alcohol added (fluctuated between + ami 



-) 



Kept in darkness i-Jo 



Kept at 15 degrees C. ! 1^.25 



1 The other line kept in pond water instead of 10 per cent, hay infusion. 



* The other line kept in darkened place to counteract darkness of refrigerator. 



