INTERACTIONS OF PROTOPLASMIC MASSES. IJ'l 



lines jd and kd to the conditions of lines j and k respectively, by 

 conveying some of the culture media in which the latter had been 

 living to the concavities containing the former. Under this 

 treatment individuals from lines j and jd reacted negatively 

 towards each other's fragments during the succeeding fifteen 

 generations (until September 9). After this cross-fusions were 

 the usual occurrences, though occasional shattering reactions were 

 encountered. When the experiment was discontinued on Novem- 

 ber i line j represented 129 generations and line jd 120 genera- 

 tions since the beginning of clone J. The reactions between 

 lines k and kd continued to be of the shattering type until 

 September 16 (for 24 generations). From then until the experi- 

 ment was discontinued on November I (line k-1^5 anc ^ h'ne 

 kd-133) most of the interactions were positive. 



(c) In these experiments specimens from clone J, lines j and jd 

 and clone K, lines k and kd were taken from the Petri dish cul- 

 tures and placed in concavities containing 10 per cent, hay 

 infusion plus i per cent, sucrose; but after remaining in this 

 solution for six days, the organisms had decreased so much in 

 si/e that they were put back in standard medium. Hvery other 

 day small quantities of culture media were exchanged between 

 the receptacles in which sister lines were kept. The organisms 

 subjected to i per cent, sucrose not only decreased in size, but 

 their physiological states were so altered that they no longer 

 fused with fragments from closely related individuals which had 

 been kept in standard culture medium see section II, (6). Thus, 

 it is evident that an environment different from what existed 

 before had been established. 



Upon testing by the usual method, it was found that the 

 shattering type of reactions persisted between specimens of line 

 j and line jd for 18 days and 19 generations, but from then on, 

 cross-fusion was exhibited. The experiment was discontinued 

 on Nov, 2d, at which time line j represented 125 generations 

 and line jd 122. In clone K shattering reactions were ob>er\r. 1 

 between line k and line kd until the 23d d.iy ,m<l i;tli o -aera- 

 tion after the experiment vvas started. Later olei -\ .itions 

 yielded a much larger proportion of shattering re.u ti<m> than 

 were encountered in any of the other experiment-, but a mmiln-r 

 of cross-fusions did take place between the two lines. When the 



