INTESTINAL PROTOZOA OF TERMITES. 211 



the day after incubation: By means of a sieve the termites 

 were -eparated from all pieces of wood and clumps of excrement 

 larger than them-elves. Then they were placed in beakers, and 

 a piece of moi-t filu-r paper was placed in each beaker so that one 

 corner ju-t touched tin- bottom. The termites would leave the 

 small pi 1 and excrement and crawl up the filu-r paper, 



and when a lar-e number of them were on the filter paper they 

 wen- -haken from it and transferred to several small beakers. 

 By tin- method the termites were entirely freed from all parti' 

 of wood .Mul ex< rement. Now they were taken, one at a time, 

 from tin -mall beaker- and placed in watch gl. Here the 



rijit antenna w close up to the head, and one by one, 



ah IT the riijit antenna had been removed, they were placed in 

 shell vials until ten individuals were in each vial. In this ex- 

 periment 14 \ial- with 10 defaunated termites each were u-ed. 

 The-e were numbered [ 1 4/749. Some unincubated and faunat ed 

 termite- were taken from jar 9p45, collected at the same time 

 that <>/ js was and taken from the same colony of termite- in 

 nature. Tin < ed from wood and excrement in the same 



way that the defaunated ones were. Then 10 unincubated and 

 faunated termite- were placed with 10 incubated and defaunated 

 termite- in \ial- i n/>49; in vials 12, 13, and !4/>4 ( ) fifty unin- 

 cubated termite- were placed. Workers only were used in all 

 the experiment-. Whatman's filter paper, moistened with di 

 tilled v put in each vial; the vials were tightly corked 



and left at rooin temperature in the dark. l\e-uh>: 1<> 2 \ 22 at 

 5 I'.M. three termites from vial 8^)49 were examined. Two con- 

 tained no protozoa and one contained a few. i<> 27 22 all of the 

 indi\idual- those previously defaunated and tlio- ( - from which 

 no pn>to/oa had been removed by incubation in ii/->4<> were 

 examined. Two were dead, 12 harbored proto/-a, and <> harbored 

 no protozoa. Three of the 12 that harbored protozoa had the 

 risjit antenna cut off. This was a clear-cut case of refaun.ition 

 (replacing of the protozoa in the inte-tine after they had been 

 removed by incubation). The number of protozoa present in 

 these refaunaud or reinfected individuals was about half normal. 

 On 12 2ii 22 six vials were examined with results as follow-: 



