" 



212 L. R. CLEVELAND. 



Number 3/749 contained 16 termites, 6 with no right antenna. 



" 4/>49 9 " , 7 



" 5/>49 16 " , 7 



" 6/>49 ii " ,5 



n ii ii i i ii ii ii 



7/>49 16 , 4 



In number 14/^49 fifty unincubated and faunated termites had 

 been placed with the ten incubated ones and only two individuals 

 with the right antenna cut off were taken from this vial, though 

 others were present and might have been taken. Nearly all of 

 the fifty unincubated individuals were alive. Now the termites 

 with the right antenna removed which were taken from vials 

 3, 4, 5, 6, 7, and i4/>49 to be counted were placed in separate vials 

 i-6/>88 with a label on each vial designating the source. What- 

 man's filter paper was given them for food. They were observed 

 regularly. All are alive now, 2/7/23, 1 five weeks from the time they 

 were separated from the unincubated. Two of the individuals 

 in 3/>49 were examined and were heavily infected with protozoa. 

 After association for several weeks with termites harboring 

 protozoa the defaunated termites regain their protozoa and, at 

 the same time, their ability to live on a diet of pure cellulose or 

 wood. Now, the important question is, how did the termites 

 regain their ability to make use of pure cellulose or wood as food? 

 More specifically, why do they now possess cellulase and, 

 perhaps, cellobiase? When the protozoa are removed from a 

 termite's intestine, the ability to make use of pure cellulose or 

 wood is lost, but when they are replaced, this ability reappears. 

 Do the cells of the termite possess cellulase and cellobiase? It 

 seems that they do not. Then where may these enzymes be 

 found? The fact that there presence can only be demonstrated 

 when the protozoa are present in the intestine of the host seems 

 to indicate that the protozoa possibly possess them. It the 

 protozoa do possess these enzymes, and the host does not, the 

 way in which they aid their host is plain. 



It seems evident that the protozoa aid their host in some w.iy 

 to live on cellulose and wood, for it has been shown (Table III.) 

 that defaunated termites can live indefinitely on the digestion 

 products of pure cellulose or wood (humus). It now 1 KV< >mes 



1 Many are alive now 4/10/23. 



